Gel electrophoresis in combination with laser ablation-inductively coupled plasma mass spectrometry to quantify the interaction of cisplatin with human serum albumin.
Identifieur interne : 000563 ( Main/Exploration ); précédent : 000562; suivant : 000564Gel electrophoresis in combination with laser ablation-inductively coupled plasma mass spectrometry to quantify the interaction of cisplatin with human serum albumin.
Auteurs : Matthew P. Sullivan [Nouvelle-Zélande] ; Stuart J. Morrow [Nouvelle-Zélande] ; David C. Goldstone [Nouvelle-Zélande] ; Christian G. Hartinger [Nouvelle-Zélande]Source :
- Electrophoresis [ 1522-2683 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antinéoplasiques, Cisplatine.
- métabolisme : Antinéoplasiques, Cisplatine.
- Antinéoplasiques, Cisplatine, Humains, Lasers, Spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antineoplastic Agents, Cisplatin.
- chemical , chemistry : Antineoplastic Agents, Cisplatin, Serum Albumin, Human.
- chemical , metabolism : Antineoplastic Agents, Cisplatin, Serum Albumin, Human.
- methods : Mass Spectrometry.
- Humans, Lasers.
Abstract
Cisplatin and its second and third generation analogues are widely used in the treatment of cancer. To study their reactions with proteins, we present a method based on SDS-PAGE separation and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for platinum detection in the reaction between human serum albumin (HSA) and cisplatin. We developed matrix-matched standards of HSA/cisplatin mixtures and used them to quantify the amount of adducts formed at different HSA:cisplatin ratios. We noted that cisplatin incubation with HSA resulted in the formation of higher order HSA n-mers, depending on the amount of cisplatin added. This caused a depletion of the HSA dimer bands, while the majority of HSA was present as the monomer. Inducing the formation of such higher molecular weight species may have an impact on the mode of action of metallodrugs.
DOI: 10.1002/elps.201900070
PubMed: 31087392
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000540
- to stream PubMed, to step Curation: 000540
- to stream PubMed, to step Checkpoint: 000550
- to stream Ncbi, to step Merge: 002217
- to stream Ncbi, to step Curation: 002217
- to stream Ncbi, to step Checkpoint: 002217
- to stream Main, to step Merge: 000566
- to stream Main, to step Curation: 000563
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Gel electrophoresis in combination with laser ablation-inductively coupled plasma mass spectrometry to quantify the interaction of cisplatin with human serum albumin.</title><author><name sortKey="Sullivan, Matthew P" sort="Sullivan, Matthew P" uniqKey="Sullivan M" first="Matthew P" last="Sullivan">Matthew P. Sullivan</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Morrow, Stuart J" sort="Morrow, Stuart J" uniqKey="Morrow S" first="Stuart J" last="Morrow">Stuart J. Morrow</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Goldstone, David C" sort="Goldstone, David C" uniqKey="Goldstone D" first="David C" last="Goldstone">David C. Goldstone</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Biological Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Hartinger, Christian G" sort="Hartinger, Christian G" uniqKey="Hartinger C" first="Christian G" last="Hartinger">Christian G. Hartinger</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31087392</idno><idno type="pmid">31087392</idno><idno type="doi">10.1002/elps.201900070</idno><idno type="wicri:Area/PubMed/Corpus">000540</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000540</idno><idno type="wicri:Area/PubMed/Curation">000540</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000540</idno><idno type="wicri:Area/PubMed/Checkpoint">000550</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000550</idno><idno type="wicri:Area/Ncbi/Merge">002217</idno><idno type="wicri:Area/Ncbi/Curation">002217</idno><idno type="wicri:Area/Ncbi/Checkpoint">002217</idno><idno type="wicri:Area/Main/Merge">000566</idno><idno type="wicri:Area/Main/Curation">000563</idno><idno type="wicri:Area/Main/Exploration">000563</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Gel electrophoresis in combination with laser ablation-inductively coupled plasma mass spectrometry to quantify the interaction of cisplatin with human serum albumin.</title><author><name sortKey="Sullivan, Matthew P" sort="Sullivan, Matthew P" uniqKey="Sullivan M" first="Matthew P" last="Sullivan">Matthew P. Sullivan</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Morrow, Stuart J" sort="Morrow, Stuart J" uniqKey="Morrow S" first="Stuart J" last="Morrow">Stuart J. Morrow</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Goldstone, David C" sort="Goldstone, David C" uniqKey="Goldstone D" first="David C" last="Goldstone">David C. Goldstone</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Biological Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Hartinger, Christian G" sort="Hartinger, Christian G" uniqKey="Hartinger C" first="Christian G" last="Hartinger">Christian G. Hartinger</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemical Sciences, University of Auckland, Auckland, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Chemical Sciences, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author></analytic><series><title level="j">Electrophoresis</title><idno type="eISSN">1522-2683</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antineoplastic Agents (analysis)</term><term>Antineoplastic Agents (chemistry)</term><term>Antineoplastic Agents (metabolism)</term><term>Cisplatin (analysis)</term><term>Cisplatin (chemistry)</term><term>Cisplatin (metabolism)</term><term>Humans</term><term>Lasers</term><term>Mass Spectrometry (methods)</term><term>Serum Albumin, Human (chemistry)</term><term>Serum Albumin, Human (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antinéoplasiques ()</term><term>Antinéoplasiques (analyse)</term><term>Antinéoplasiques (métabolisme)</term><term>Cisplatine ()</term><term>Cisplatine (analyse)</term><term>Cisplatine (métabolisme)</term><term>Humains</term><term>Lasers</term><term>Spectrométrie de masse ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antineoplastic Agents</term><term>Cisplatin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antineoplastic Agents</term><term>Cisplatin</term><term>Serum Albumin, Human</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antineoplastic Agents</term><term>Cisplatin</term><term>Serum Albumin, Human</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antinéoplasiques</term><term>Cisplatine</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mass Spectrometry</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antinéoplasiques</term><term>Cisplatine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Lasers</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antinéoplasiques</term><term>Cisplatine</term><term>Humains</term><term>Lasers</term><term>Spectrométrie de masse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cisplatin and its second and third generation analogues are widely used in the treatment of cancer. To study their reactions with proteins, we present a method based on SDS-PAGE separation and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for platinum detection in the reaction between human serum albumin (HSA) and cisplatin. We developed matrix-matched standards of HSA/cisplatin mixtures and used them to quantify the amount of adducts formed at different HSA:cisplatin ratios. We noted that cisplatin incubation with HSA resulted in the formation of higher order HSA n-mers, depending on the amount of cisplatin added. This caused a depletion of the HSA dimer bands, while the majority of HSA was present as the monomer. Inducing the formation of such higher molecular weight species may have an impact on the mode of action of metallodrugs.</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li></country></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Sullivan, Matthew P" sort="Sullivan, Matthew P" uniqKey="Sullivan M" first="Matthew P" last="Sullivan">Matthew P. Sullivan</name></noRegion><name sortKey="Goldstone, David C" sort="Goldstone, David C" uniqKey="Goldstone D" first="David C" last="Goldstone">David C. Goldstone</name><name sortKey="Hartinger, Christian G" sort="Hartinger, Christian G" uniqKey="Hartinger C" first="Christian G" last="Hartinger">Christian G. Hartinger</name><name sortKey="Morrow, Stuart J" sort="Morrow, Stuart J" uniqKey="Morrow S" first="Stuart J" last="Morrow">Stuart J. Morrow</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000563 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000563 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31087392
|texte= Gel electrophoresis in combination with laser ablation-inductively coupled plasma mass spectrometry to quantify the interaction of cisplatin with human serum albumin.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31087392" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |